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By Benjamin F. Paley, Tomas E. Warfield

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2003). Subunit-dependent effects of nickel on NMDA receptor channels. Brain Res Mol Brain Res, 117, 139-44. Marchetti, C. & Gavazzo, P. (2005). NMDA receptors as targets of heavy metal interaction and toxicity. Neurotoxicity Research, 8, 1-14. Paoletti, P. & Neyton, J. (2007). NMDA receptor subunits: function and pharmacology. Curr Opin Pharmacol, 7, 39-47. , Hua, Y. , Chen, Y. Z. & Luo, J. H. (2005). Subunit assembly of Nmethyl-d-aspartate receptors analyzed by fluorescence resonance energy transfer.

2004). , 2002, 2003). Thus, it seems that the L-Glu effect on Ach release, increasing or decreasing it, must depend on the L-Glu receptor subtype expressed by the neuronal type. 4. Dopamine Release Dopamine is a neurotransmitter involved in adaptation to a photopic condition. , 2000). , 2001; 2003). , 2007). , 1980; Tapias & Arias, 1982). Depolarization induced by high KCl 28 Roberto Paes-de-Carvalho, Karin da Costa Calaza, Marcelo Cossenza et al. , 1980; Tapias & Arias, 1982). On the other hand, L-Glu and kainate does not affect dopamine release (Tapias & Arias, 1982).

2). This approach proved fruitful also for the task of characterizing antibody specificity for subsequent use in primary culture. Conclusion Our study demonstrates that electroporation can be employed successfully to study NRs at single cell level. Although an initial investment is required to buy the electroporator unit, after that the procedure is very inexpensive and reproducible because the involved variables are easily controlled. In particular, the use of a physiological saline during the voltage pulse instead of culture medium contributed a significant enhancement in cell survival, while identification of double transfected cells was improved by minimizing the concentration of A Rapid and Efficient Protocol for Transfecting NMDA Receptor Channels… 15 the control CD8 DNA.

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