Download A Sand County Almanac (Outdoor Essays & Reflections) by Aldo Leopold PDF

By Aldo Leopold

"We can position this publication at the shelf that holds the writings of Thoreau and John Muir." San Francisco Chronicle

These awesome pix of the flora and fauna discover the breathtaking range of the unspoiled American panorama -- the mountains and the prairies, the deserts and the coastlines. a gorgeous tribute to our land and a daring problem to guard the realm we like.

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Additional info for A Sand County Almanac (Outdoor Essays & Reflections)

Sample text

We therefore concentrated on the second possibility and examined the efficiency of operation of the pump. This is also not a simple matter and we adopted an approach which was used several years ago by Whittam and Ager (23). Since glycolysis is a tightly coupled process, one mole of ADP must be converted to ATP for each mole of lactate formed. Provided the supply of ADP is derived from the hydrolysis of ATP by the plasma membrane Na+K+-ATPase, we can calculate how many molecules of K+ or Rb+ are taken up for each lactate formed.

The titer of the extract shown is taken as 1/64 (greatest dilution that produces agglutination). 1 M] completely inhibits agglutination; but an identical concentration of N-acetylD-glucosamine has no effect. The assay may be performed in two ways as described (20, 21). bands on polyacrylamide gel electrophoresis in a sodium dodecy1 sulfate system, a major band containing more than 90% of the stained protein and a minor band of slightly lower molecular weight containing the remainder (25). The purified proteins, called discoidin I and II (10), will be discussed in more detail by Frazier et a1.

Effect of sugars on aggregation of cohesive P. pa11idum cells. Cohesive P. pa11idum cells were gyrated in suspension and agglutination was-monitored by determining disappearance of single cells with a quantitative assay using a Coulter counter in the presence and absence of the indicated concentration of sugars. Two separate experiments are shown. For details see ref. 21. employing a Coulter counter. Association constants and numbers of binding sites were determined for the binding of all three 1ectins to both Q.

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